Volume-6, Number-1, February-2013



Res. Environ. Life Sci., 6(1) 1-4 (2013)


Impact of urban wastes on the physico-chemical

characteristics of river Gomti at Lucknow

Radheyshyam Sahu1,K. A.Gupta1, Neeraj Kumar Agarwal2, Shradha Sinha2

1Department of Chemistry, Hindu P.G. College, Moradabad

2Department of Chemistry, BBDNITM, Lucknow


(Received: March 16, 2012; Revised received: October 03, 2012;Accepted: October 06,2012)


(Download full paper)


Abstract: Gomti river receives industrial as well as domestic wastes from various drains of Lucknow city. In this process the water of river Gomti gets contaminated with heavy metals and other pollutants. In the present study impact of above wastes on the Gomti river water have been investigated from January 2009 to June 2009. For this, six sampling sites were selected and the samples of water were collected and analyzed month wise. The results indicate that the pH of Gomti water is under acceptable limit of water quality. Chemical oxygen demand at one site is higher than WHO permissible limit. Therefore water should be treated before use of various purposes’. All the other parameters like chloride, sulphate, alkalinity, chromium and lead are under permissible limits..

Key words: Gomti river water, Fluoride, Alkalinity, Dissolved oxygen and Metals


Res. Environ. Life Sci., 6(1) 5 -8 (2013)


Hospital solid waste and waste water analysis in Lucknow

Reetanjali Singh*, Shashank Shekhar Mishra, Ajai Mishra

Centre of Advanced Study in Geology, University of Lucknow, Lucknow-226007, India


(Received: April 15, 2012; Revised received: September 13, 2012;Accepted: September 19,2012)


(Download full paper)



Abstract: Biomedical waste is generated in hospitals, research institutions, health care teaching institutes, clinics, laboratories, blood banks, animal houses and veterinary institutes. These wastes have potential to spread dangerous diseases if disposed indiscriminately. The study reveals the existing scenario of different types of clinical wastes alongside the domestic wastes and primary results on the physico - chemical properties of the hospital waste water before their discharge in the municipal sewerage system and their effects on the environment. It was observed that the major producer of bio-medical waste is KGMC i.e. on an average 2195 quintal/year where as the minimum producer of bio-medical waste is Sanjivani Medical Center and Javitri Nursing Home i.e. 33.50 quintal respectively. As per the estimation and survey the total biomedical waste generation in Lucknow is more than 2.0 MT/day. The effluents present an important concentration and high BOD, COD and Suspended solids values showed that water is highly contaminated.


Key words: Biomedical waste management, hospital wastewater, and urban environment



Res. Environ. Life Sci., 6(1) 9-10 (2013)


Isolation and identification of cellulolytic bacteria from gut of termites

S. Priya*1and P. Maheswari 2

1Department of Biotechnology, 2Department of Bioscience, S.T.E.T. college of Education for women, Mannargudi, India


(Received: May 21, 2012; Revised received: October 12, 2012; Accepted: October 15, 2012)


 (Download full paper)


Abstract: The present research was to isolate and identify cellulolytic bacteria from the gut region of the local termites Odontotermes obesus. The isolates were cultures in a medium containing carboxy methyl-cellulose and cellobiose. The bacterial species were tentatively identified by using the Bergey’s manual. The species were all novel strains and identified as Bacillus sp.,Pseudomonas and Cellulomonas sp. respectively.


Key words: Isolation, Bacillus sp., Pseudomonas and Cellulomonas sp.


Res. Environ. Life Sci., 6(1) 11 -12 (2013)


Screening of genotype for bacterial leaf blight on rice

Prabhat Kumar Singh

Postgraduate Department of Botany, Kisan P. G. College, Bahraich, India


(Received: October 15, 2012; Revised received: January 18, 2013; Accepted: January 19, 2013)


(Download full paper)



Abstract: Bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae is one of the most destructive disease in majority of rice growing areas. The use of resistant variety is best method to manage the disease. Therefore 75 genotype have been screened during 20011-12 cropping season. For varietal screening each genotype were sown in well prepared pots in the second week of July 2011. Out of seventy five genotype screened none have been found to be free from the disease, three genotype viz; Malviya Dhan 36, Sarjoo 52, and Gorakhnath were resistant, twenty three genotype viz; IR-20, IR-36, IR-64, Swarndhan, Pusa Basmati-1, Samba Mehsuri, Radha, HKR 95-131, 6444, Shankardhan, Poineer, Dhanrekha, Narendra Usar dhan-2, Narendra Usar dhan-3, Pant Dhan-11, Chaini, IRBB-4, B-60, B-170, IRBB-60, Madhuri, Chaurasia weremoderately resistant, twenty nine genotype were moderately susceptible, seventeen genotype were susceptible and only three genotype viz; Rajshree, Dhanlakshmi and Padmini were rated as highly susceptible. The genotype showing consistently high degree of resistance may be utilized for the development of high yielding disease resistant varieties.

Key words: Screening, Genotype, Bacterial leaf blight, Rice



Res. Environ. Life Sci., 6(1) 13 -16 (2013)


Genetic variability assessment among Syzygium cumini Skeels based on dominant and co-dominant DNA marker systems

Jitendra P. Singh*1, A.K. Singh 1, Anju Bajpai 1, Iffat Zareen Ahmad 2 andMuthukumar. M.1

1Division of Crop Improvement and Biotechnology, Central Institute for Subtropical Horticulture,

Rehmankhera, Lucknow - 226 101 (U.P.), India

2Department of Biotechnology, Integral University, Dasauli, Kursi Road, Lucknow-226 026, Uttar Pradesh, India



(Received: October 03, 2012; Revised received: January 20, 2013; Accepted: January 22, 2013)


(Download full paper)



Abstract: Syzygium cumini Skeels (Jamun), known for its nutraceutical and antidiabetic properties, exhibits appreciable variability in their natural population.The genetic variability among the jamun accessions were assessed using dominant (randomly amplified polymorphic DNA - RAPD) and co-dominant (direct amplification of minisatellite DNA - DAMD, simple sequence repeats -SSRs) marker systems. A total of 117 fragments were generated across the eleven accessions with 92 being polymorphic. The number of bands produced by each primer varied from 6 (OPB-12 and OPG-13) to 17 (OPG 3) with an average of 10.63 per primer. The cluster analysis through UPGMA method produced a dendogram depicting genetic relatedness among the jamun accessions based on molecular diversity in which the accessions were grouped into three clusters. The distance matrix generated by Jaccard’s genetic distance coefficient ranged between 0.488 (CISH J-42 and PKM) to 0.788 (J -34, J-37 and PKM). Among four minisatellite primers tested, 33.6 primer displaying 85.71 % polymorphism. Out of ten SSR primers pairs the two primer viz., mPgCIR 15 and mPgCIR 16 exhibited cross species amplification, however, multi-allelic banding was exhibited limiting its use in characterization. Transferability of guava SSR markers was confirmed validating highly conserved regions among jamun accessions.


Key words:Syzygium cumini Skeels, RAPD, SSR, DAMD and cross species amplification


Res. Environ. Life Sci., 6(1) 17 -18 (2013)


Concept on transmission of mung bean mosaic virus (MBMV) through aphids

Brij Lal Verma2, Dr. B.D.Singh1

1Department of Botany, K.S. Saket P.G. College, Ayodhya, Faizabad (UP) India, 224123

2Dr. R.M.L. Avadh University Faizabad (UP) India


(Received: September 02, 2012; Revised received: January 01, 2013; Accepted: January 04, 2013)


(Download full paper)



Abstract:An experiment was conducted during-2010 under the supervision of first author to see the mode of transmission of mung-bean mosaic virus through Aphids. The Mung bean plant belongs to the family leguminosae and sub family papilionaceae. It is mainly grown for its seeds which are highly nutritious and are used as pulse (dal), delicious preparations, cattle feed. The crop is very commonly affected by mung bean mosaic virus (MBMV) which are transmitted by cell sap and Aphids.


Key words:Transmission, Aphids, Leguminosae,Symptoms, Chlorosis, Inoculum, Acquisition feeding


Res. Environ. Life Sci., 6(1) 19 -22 (2013)


Diurnal fluctuations in population density of Copepods from Yassbolagh Dam, Markazi, Iran

Alireza Shayestehfar*¹,Samaneh Abdoveis²andSima Aslanfaal

1Department of Biology, Faculty of Science, Arak University -38156-8-7349, Iran

2Department of Basic Sciences of Water Resources, Khuzestan Water and Power Authority, Ministry of Energy, Iran


(Received: April 04, 2012; Revised received: January 05, 2013; Accepted: January 18, 2013)


(Download full paper)



Abstract: The free living copepods are considered as zooplanktons and consume as food by fishes. In the present study diurnal fluctuations in population density of copepods in relation to temperature, dissolved oxygen, pH, acidity and alkalinity were studied in Yassbolagh Dam which is situated in North West to Arak city. The study covered four diel periods in three months of summer, 2008. The Copepods are a sub-class of the class Maxiliopoda, sub phylum Crustacean and phylum Arthropoda, and in this study they were represented by 8 genus, 11 species and three orders (Cyclopoida, Calanoida and Harpacticoida) respectively. Maximum population density observed in D. lintoni . The high population density of copepoda in 12 and 18 possibly is due to their diurnal habitat, more water temperature, and relatively moderate oxygen concentration.

Key words: Diurnal fluctuation, Copepods, Physical and chemical parameters


Res. Environ. Life Sci., 6(1) 23-26 (2013)


Formulation and evaluation of floating nateglinide tablets using factorial design

Om prakash*, S. Saraf, M. Rahman, Neeraj Agnihotri, Vinay Pathaka

Department of Industrial chemistry, Integral University,Lucknow


(Received: March 18, 2012; Revised received: October 03, 2012;Accepted: October 04,2012)


(Download full paper)



Abstract: Nateglinide is a novel, highly physiologic, glucose regulator recently approved for the treatment of type-2 diabetes mellitus. Nateglinide has a rapid onset and short duration of insulinotropic action that results in reduction ofglucose level. The purpose of this study to develop a floating tablet of Nateglinide to enhance its bioavailability and sustained action. In the present study a 3² full factorial design was employed in which 2 factors were evaluated at 3 levels, experimental trials were performed at all possible 9 combinations. The independent variables selected for this study were concentration of Ethyl cellulose (X1) and sodium bicarbonate (X2).% drug release for 30min,1h, 2h,4h,6h, 8h,12h, 16h,24h and floating lag time (FLT) were as dependent variables. The results of factorial design showed that factor X1 and X2 significantly affect the studied dependent variables. The floating tablet formulations were evaluated for Bulk density (gm/cm3), Tapped density(gm/cm3), Hausner ratio(HR), Carr index, Angle of repose, flow property, assay, in-vitro drug release, hardness, friability,weight variation. The results of in vitro release studies showed that the optimized formulation (F8) could sustain drug release (98.33%) for 24h. Stability of tablets at 400C/75%RH, of optimized formulation was carried for one month and no significant change was observed.


Key words: Nateglinide, 3² full factorial design, floating tablet, Ethyl cellulose (X1) and sodium bicarbonate (X2), sustain drug release (98.33%) for 24h



Res. Environ. Life Sci.,6(1) 27-34 (2013)


Diversity of potent ethnomedicinal plants from Bahraich (U.P.), India

SangeetaSahani and T. P. Mall

Postgraduate Department of Botany, Kisan PG College Bahraich – 271 801 (U. P.)



 (Received: September 21, 2012; Revised received: January 22, 2013;Accepted: January 24, 2013)


 (Download full paper)



Abstract: The present study revels about the vast diversity of herbal plants used by tribes as well as poor villagers of Bahraich district. The potential of ethnobotanical research and need for documentation of traditional knowledge pertaining to the medicinal plant utilization for the greater benefit of mankind is carried out because most of the villages of Bahraich district are not having sufficient medical facilities. But all the villages have the traditional medicines and treatments to cure all the ailments. The rural population has to depend on the local ethnic doctor called as viaday or hakim. They prepare medicines from the medicinal plants available in their locality. They follow all the traditional and ethnic method of preparing the medicine. Bahraich has well blessed phytodiversity which is a rich source of medicinal plants as well as ethnic communities. The remote locality, poverty illiteracy and lack of touch with modern civilization make them confined to hold on traditional faith hence they are wholly dependent on indigenous plants for the treatment of various ailments. The investigation was performed by collection of local medicinal plants in consultation with the local tribes as well as poor villagers. The common medicinal plants used by rural tribes were studied and about seventy six plant species belonging to sixty nine genera representing thirty six families were found to be utilized in the treatment of various twenty five ailments viz. antidote for snake and scorpion bite, arthritis, burn, cough, cold, coryza, constipation, conjunctivitis, diabetes, ear pain, fever, gastrointestinal troubles, headache, insomnia, jaundice, leucorrhoea, mouth ulcer, respiratory troubles, skin problems and skeletal problems etc. Various medicinal plant parts viz. leaves, flowers, bark, latex, seeds, rhizome, root, sometimes whole plant were used as ethnomedicine. For each plant species, details on scientific name, authors name, vernacular name, family name, as well as use were provided along with parts harvested for treatment and the mode of administration.


Key words: Ailments, Ethnobotanical, Ethnomedicine, Medicinal plants, Phytodiversity, Secondary metabolites, Traditional knowledge, Bahraich



Res. Environ. Life Sci., 6(1) 35-36 (2013)


Study on blight disease of pumpkin at alan Dist. Sultanpur U.P.

Nikhil Kumar Dubey*, .Alok Kumar Pandey and Sahab Lal Yadav

Dept. of Botany and Plant Pathology, Shri Vishwanath P.G. College Kalan Sultanpur U.P. India

*e-mail: -

(Received: September 12, 2012; Revised received: January 28, 2013; Accepted: January 29, 2013)


(Download full paper)


Abstract: Blight implies a sudden and extensive damage of leaves. In many leaf diseases the area of necrosis is limited which gives the appearance of spot. Pumpkin is major vegetable crop in District Sultanpur. The disease is sporadic in Sultanpur and nearby districts. The fungus occurs naturally in most soils and can infect pumpkin at most stages of growth when there is excess soil moisture and warm, wet weather. The fungus overwinters in soil as thick-walled oospores. It has been described as the ‘most destructive disease of cucurbits’ because ‘nothing causes greater loss’. Total crop loss may occur in some fields. The present study is made to find out disease cycle occurrence and mode of prevention of pumpkin blight disease.

Key words: Blight, Pumpkin, fungus, sporadic